| 摘要: |
| 鱼类浮游生物的准确鉴定是鱼类浮游生物研究的基础。传统的基于形态特征的鉴定不仅费时费力，而且由于缺乏足够信息，鉴定存在困难，导致物种多样性被低估。为了对物种进行准确、快速地鉴定，急需在传统形态分类学基础上，建立并结合便捷准确的分子鉴定手段。DNA 条形码技术是利用一段较短的DNA 序列实现快速准确物种鉴定的工具，就像在商店里扫描仪读取条形码那样,对每一种生物也能通过快速分析其DNA 中的一小段(线粒体细胞色素c 氧化酶I亚基,mt COI) 加以识别。DNA条形码提供了可信息化的分类标准和有效的分类学手段，已成为近年来生物类群的研究热点。文章介绍了DNA 条形码的概念、原理、工作流程及其优点，分析了其在鱼类浮游生物鉴定中的应用可行性，并展望未来鱼类浮游生物学发展的前景：分子技术鉴定鱼类浮游生物相关规范标准的建立，传统形态鉴定与分子方法相结合的分类学研究，以及鱼类浮游生物的生态学研究。 |
| 关键词: DNA条形码 鱼类浮游生物 形态分类 物种鉴定 |
| DOI： |
|
| 基金项目:海洋公益性行业科研专项(201305027,201505004-6 )；国家海洋局东海分局青年海洋科技基金项目(201608). |
|
| Review and Outlook of DNA Barcoding in Identification of Ichthyoplankton |
| LIU Shouhai,CHENG Xiangsheng,HE Yanlong,QIN Yutao |
| East China Sea Environmental Monitoring Center,State Oceanic Administration;Key Lab of Integrated Monitoring and Applied Technology for Marine Harmful Algal Blooms,State Oceanic Administration |
| Abstract: |
| As the main components of marine biota,ichthyoplankton plays vital roles in the marine biodiversity.However,morphological identification of ichthyoplankton was timeconsuming and even impossible for some taxa.Diversity of marine ichthyoplankton was believed to be underestimated.To enable accurate and fast identification of species,it is imperative to establish molecular techniques,to complement the traditional morphological methodology.DNA barcoding (a technology to provide rapid，accurate and automated species identifications by using short orthologous DNA sequences ) had been fundamentally important in the past few years in classification.Just like the barcodes on products are recognized,each living species can be identified by using a short fragment of the mitochondrial gene coding for cytochrome c oxidase subunit I (mt COI).DNA barcoding provides digitalized criteria and effective means for species identification.This paper reviewed some fundamental knowledge of DNA Barcoding,like the conception,principle,workflow and advantages of DNA barcoding.Meanwhile,the possibility and feasibility of DNA barcoding in identification of ichthyoplankton were discussed.And the outlook of ichthyoplankton was prospected,like the establishment of molecular standards for identification of ichthyoplankton,a taxonomic study combining morphological identification with molecular methods,ecological studies of ichthyoplankton. |
| Key words: DNA barcoding,Ichthyoplankton,Morphological taxonomy,Species identification |
