| 摘要: |
| 对物种准确鉴定是进行生物多样性研究的前提。为提高物种鉴定的准确性，文章采用DNA条形码技术结合形态学对长江口仔稚鱼样品进行种类鉴定。结果表明，44个样品中，通过线粒体COI基因的分析鉴定有17个种、1个属；部分形态上难以鉴定的种类，如虾虎鱼科的矛尾虾虎鱼、斑尾刺虾虎鱼、睛尾蝌蚪虾虎鱼、褐吻虾虎鱼和斑点竿虾虎鱼，可通过DNA条形码实现有效鉴定；形态较为相似的种类，通过DNA条形码可鉴定到种，淡水鲤科的有青鱼、鲢、鳊，鲻科的有龟鮻和前鳞龟鮻。利用DNA条形码技术结合形态进行仔稚鱼种类的鉴定，能提高仔稚鱼鉴定的精确度。 |
| 关键词: DNA条形码 生物多样性 COI基因 海洋生物 物种鉴定 |
| DOI： |
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| 基金项目:海洋公益性行业科研专项(201305027,201505004-6);海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室基金资助课题(MATHAB201402,MATHAB201408);东海分局青年海洋科技基金项目(201501,201608). |
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| Molecular Identification of Fish Larvae and Juveniles Based on DNA Barcoding |
| LIU Shouhai,QIN Yutao,LIU Caicai,JI Xiao,ZHANG Haofei |
| East China Sea Environmental Monitoring Center，SOA;Key Lab of Integrated Monitoring and Applied Technology for Marine Harmful Algal Blooms，SOA |
| Abstract: |
| Accurate identification of species is a prerequisite for biodiversity research.To improve the identification accuracy of fish larvae and juveniles in the Yangtze Estuary，the method of morphology and DNA barcoding were used based on mitochondrial the COI gene.The results showed that among the 44 samples analyzed，17 of which could be identified to the species level using DNA barcoding，while only one to the genus level.Some species without available taxonomic keys for morphological identification，including Chaeturichthys stigmatias，Acanthogobius ommaturus，Lophiogobius ocellicauda，Rhinogobius brunneus，Luciogobius guttatus in Gobiidae，were difficult to identify by morphology but were successfully identified by using DNA barcoding.Some species with similar morphology were hard to be distinguished，such as Mylopharyngodon piceus，Hypophthalmichthys molitrix，Parabramis peknensis in Freshwater Cyprinidae，Chelon haematocheilus，Chelon affinis in Mugilidae.DNA barcoding based on the COI gene could provide effective identification to the species level.Collectively，DNA barcoding，together with morphological identification，could largely increase the identification accuracy for fish larvae and juveniles. |
| Key words: DNA barcoding,Biodiversity,COI gene,Marine organism,Species identification |
