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  • 张添,隋宥珍,刘连为,孟玮,徐开达,邹锟,周永东.基于环境DNA技术的舟山南部海域鱼类资源状况和多样性分析[J].海洋开发与管理,2024,41(3):114-120    
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基于环境DNA技术的舟山南部海域鱼类资源状况和多样性分析
张添,隋宥珍,刘连为,孟玮,徐开达,邹锟,周永东
浙江海洋大学海洋与渔业研究所;浙江省海洋水产研究所 农业农村部重点渔场渔业资源科学观测实验站 浙江省海洋渔业资源可持续利用技术研究重点实验室浙江省海水增养殖重点实验室
摘要:
为完善舟山南部海域鱼类种类组成及结构特征,文章使用环境DNA技术对该海域鱼类多样性进行检测。通过采集水样、过滤、提取环境DNA、PCR扩增、测序,在该海域6个站位中获得8个环境DNA样品,共检测到9种舟山海域常见鱼类。其中,软骨鱼纲1种,辐鳍鱼纲8种,龙头鱼(Harpadon nehereus)为优势种。研究结果表明,虽然环境DNA技术无法完全替代传统的鱼类资源调查方法,但是可以作为鱼类资源调查和空间分布的补充手段,与底拖网数据结合,为舟山南部海域鱼类组成和群落结构季节性变化提供依据。
关键词:  环境DNA  物种组成  鱼类多样性  资源调查
DOI:10.20016/j.cnki.hykfygl.2024.03.003
投稿时间:2023-06-17修订日期:2024-01-22
基金项目:国家重点研发计划项目(2019YFD0901204, 2020YFD0900804);浙江省重点研发计划项目(2019C02056);浙江省渔业资源调查专项(HYS-CZ-202314).
Analysis on the Status and Diversity of Fish Resources in the Southern Sea Area of Zhoushan Based on Environmental DNA Technology
ZHANG Tian,SUI Youzhen,LIU Lianwei,MENG Wei,XU Kaida,ZOU Kun,ZHOU Yongdong
Marine and Fisheries Institute of Zhejiang Ocean University;Marine and Fisheries Institute of Zhejiang Ocean University; Zhejiang Marine Fisheries Research Institute, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds, Ministry of Agriculture and Rural Affairs of the People′s Republic of China, Key Laboratory of Sustainable Utilization of Technology Research for Fisheries Resources of Zhejiang Province, Zhejiang province Key Lab of Mariculture and Enhancement
Abstract:
In order to improve the species composition and structural characteristics of fish in the southern sea area of Zhoushan, the environmental DNA technology was used to detect the fish diversity. By collecting water samples, filtering, extracting environmental DNA, PCR amplification and sequencing, 8 environmental DNA samples were obtained from 6 stations in the southern sea area of Zhoushan, and 9 species of common fish in Zhoushan sea area were detected, including 1 species of cartilaginous fish, 8 species of radial fin fish, and Harpadon nehereus was the dominant species. The results show that, although the environmental DNA technology can not completely replace the traditional methods of fish resources investigation, it can be used as a supplementary means of fish resources investigation and spatial distribution. Combined with bottom trawl data, it provides a basis for the seasonal changes of fish composition and community structure in the southern sea area of Zhoushan.
Key words:  Environmental DNA, Species composition, Fish diversity,Survey of resource