摘要: |
为探讨小球藻鉴定的新方法以及评估小球藻属 DNA 条形码候选序列的鉴定作用,文章对20株小球藻的线粒体基因细胞色素C氧化酶亚基Ⅰ(COI)、核基因组rDNA的内转录间隔区(ITS)以及核酮糖1,5-二磷酸羧化酶(rbcL)3个片段进行PCR扩增测序,并对各序列进行生物信息学分析。研究结果表明:COI、ITS和rbcL序列在20株小球藻中的扩增和测序效率均呈阳性,扩增成功率分别为76%、83%和70%;经评估,COI、ITS和rbcL序列作为DNA条形码在小球藻分类鉴定中均不适合较低的分类单位,但可参考动植物的分类方法,将多个片段组合应用,从而筛选不同进化级别的DNA条形码。 |
关键词: 小球藻 DNA条形码 分类鉴定 物种 基因 |
DOI: |
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基金项目:自然资源部第三海洋研究所基本科研业务费专项资金资助项目(海三科2015012、海三科2019015). |
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Assessment of Candidate DNA Sequences for Barcoding of the Genus Chlorella |
ZHANG Zhilan,XING Bingpeng |
Third Institute of Oceanography,MNR;Observation and Research Station of Coastal Wetland Ecosystem in Beibu Gulf,MNR |
Abstract: |
To evaluate the validation of DNA barcoding candidate sequence in identifying Chlorella,to discuss the new ways of identifying,this paper took 20 individuals sampled for analysis,3 sequences cytochrome coxidase Ⅰ(COI),internal transcribed spacer (ITS) and ribulose1,5-bisphosphate of carboxylase (rbcL) were amplified and sequenced.These sequences were analyzed by bioinformatics methods.The result showed both amplifying and sequencing effects of COI,ITS and rbcL towards the sample of chlorella were positive,the amplification success rate was 76%,83% and 70%,these 3 sequences were not suitable for lower taxa level.In the future the combination of many segments could be considered and tested,to pick out the different evolutionary levels of DNA barcoding. |
Key words: Chlorella,DNA barcoding,Identification,Species,Gene |